金克隆生物公司位于北京經濟技術開發區，是一家集研發、銷售和服務于一體的高新技術企業。擁有五千平米的研發和倉儲基地，自產試劑及常備生化試劑耗材等近兩萬種現貨庫存。涵蓋細胞學、分子生物學、蛋白免疫學、組織學、微生物培養等。
我公司金克隆有專業的技術團隊和質檢團隊。所有產品，技術團隊負責全程的技術指導分析。每個產品，質檢團隊反復檢測，合格后才會入庫銷售，出售前質檢充分，免除客戶質量之憂。

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1 細胞學：細胞培養、細胞分離、細胞檢測等各種細胞實驗的試劑耗材盒子。
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6 生化檢測試劑盒：現有26個系列,用途涵蓋醫、藥、農、林、畜牧、水產、體育、生物、環保、營養、食品衛生、預防醫學、保健品及化妝品等。
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金克隆生物公司位于北京經濟技術開發區，主要從事生物醫藥試劑的研發和生產，并兼營部分生化試劑和儀器耗材。其中我公司自主開發的分子生物學試劑、細胞培養及檢測試劑、分子免疫學產品等在質量和價格方面具有較大的市場優勢，歡迎廣大經銷商洽談合作。

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GcloneGelGreen Nucleic Acid Gel Stain (10000×)

Catalog Number: AA0115

Size: 0.5 mL

Storage:

GcloneGelGreen is stable for at least one year when protected from light, at room temperature. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50℃ for two minutes and vortex.

Product Description:

GcloneGelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GcloneGelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm. Thus, GcloneGelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).

GcloneGelGreen is far more sensitive than SYBR® Safe. Unlike SYBR® dyes, which are known to be unstable, GcloneGelGreen is very stable, both hydrolytically and thermally. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I, GcloneGelGreen is noncytotoxic and nonmutagenic at concentrations well above the working concentrations used in gel staining, because of the dye’s inability to cross cell membranes.

Gel staining with GcloneGelGreen is compatible with downstream applications such as gel extraction and cloning. It is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation. But it is not designed for qPCR applications, for which we recommend EvaGreen dye.

Staining Protocols:

Because nucleic acid binding dyes can affect DNA migration during electrophoresis, post-staining of gels is highly recommended. Post-staining with GcloneGelGreen results in superior sensitivity and eliminates the possibility of dye interference with DNA migration. Post-staining with GcloneGelGreen is simple, requiring no destaining and no special buffer. GcloneGelGreen also can be included in agarose gels using the precast method. While the precast protocol is more convenient, some DNA samples may experience migration retardation or compromised resolution in the presence of GcloneGelGreen. Thus, the post-staining and precast protocols should be compared to determine which one better meets your needs.

Although GcloneGelGreen has undergone extensive safety testing, we recommend following universal safety precautions when working in the laboratory.

1. Post-staining Protocol

1.1 Run gels as usual according to your standard protocol.

1.2 Dilute the GcloneGelGreen 10000× stock reagent 3300 fold to make a 3× staining solution in H2O.

Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye

precipitation if the gel stain is reused.

1.3 Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3× staining solution to submerge the gel.

1.4 Agitate the gel gently at room temperature for 30 minutes.

1.5 Image the stained gel with a 254 nm transilluminator, a Dark Reader® or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

1.6 Staining solution can be reused at least 2-3 times. Store staining solution at room temperature protected from light.

2. Pre-cast protocol

2.1 Prepare molten agarose gel solution using your standard protocol.

2.2 Dilute the GcloneGelGreen 10000× stock reagent into the molten agarose gel solution at 1:10000 and mix thoroughly. GcloneGelGreen can be added while the gel solution is still hot.

2.3 Cast the gel and allow it to solidify. Any leftover gel solution may be stored and re-heated later for additional gel casting. GcloneGelGreen precast gels may be stored at 4℃ for later use.

2.4 Load samples and run the gels using your standard protocol.

2.5 Image the stained gel with a 254 nm transilluminator, a Dark Reader® or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

2.6 Note: The pre-cast protocol is not recommended for polyacrylamide gels. Use the post staining protocol for acrylamide gels.