CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和Z優(yōu)培養(yǎng)條件！
CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞
|
ATCC® Number:
|
CRL-1592?
|
Price:
|
|
|
Designations:
|
IEC-6
|
|
Depositors:
|
A Quaroni
|
|
Biosafety Level:
|
1
|
|
Shipped:
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
Growth Properties:
|
adherent
|
|
Organism:
|
Rattus norvegicus (rat)
|
|
Morphology:
|
epithelial
|
|
Source:
|
Organ: small intestine
Tissue: epithelium
Disease: normal
|
|
Permits/Forms:
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
Applications:
|
transfection host (Roche FuGENE® Transfection Reagents)
|
|
Gender:
|
male
|
|
Comments:
|
Cell growth is inhibited by cortisol.
The cells express antigens characteristic of intestinal epithelia. [22491]
The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
|
|
Propagation:
|
ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
|
|
Subculturing:
|
Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
|
|
Preservation:
|
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
|
Related Products:
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
|
|
References:
|
1079: Quaroni A, et al. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc. Natl. Acad. Sci. USA 75: 5548-5552, 1978. PubMed: 103096
22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453
48719: Quaroni A, et al. Keratinexpressionin rat intestinal crypt and villus cells. J. Biol. Chem. 266: 11923-11931, 1991. PubMed: 1711043
48720: Dignass AU, Podolsky DK. Interleukin 2 modulates intestinal epithelial cell function in vitro. Exp. Cell Res. 225: 422-429, 1996. PubMed: 8660931
48721: Weiser MM, Quaroni A. A vitamin D-related inhibition of growth of an epithelioid cell line derived from rat small intestine. Biochem. Biophys. Res. Commun. 90: 788-793, 1979. PubMed: 508345
48722: Jakobs ES, et al.expressionof sodium-linked nucleoside transport activity in monolayer cultures of IEC-6 intestinal epithelial cells. J. Biol. Chem. 265: 22210-22216, 1990. PubMed: 2266122
48723: Conteas CN, Majumdar AP. The effects of gastrin, epidermal growth factor, and somatostatin on DNA synthesis in a small intestinal crypt cell line (IEC-6). Proc. Soc. Exp. Biol. Med. 184: 307-311, 1987. PubMed: 2881310
|
|
上海復(fù)祥生物提供 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和Z優(yōu)培養(yǎng)條件,網(wǎng)站上有細胞照片,聯(lián)系郵箱xiangfbio@163.com.詳情請登陸http://www.xiangbio.com/歡迎各位老師來電咨詢!
iec-6 大鼠小腸隱窩上皮細胞
馬-達二氏犬腎細胞系 MDCK (NBL-2) CRL-34
鴨子胚胎成纖維細胞 Duck embryo CCL-141
小鼠胚胎成纖維細胞 3T3-L1 CCL-173
人結(jié)腸直腸腺癌 COLO 320DM CCL-220
人結(jié)腸直腸腺癌 NCI-H508 CRL-253
人結(jié)腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細胞 Ramos（RA1） CRL-1596
人淋巴母細胞 T2(174×CEM.T2) CRL-1992
大鼠心肌細胞 H9C2(2-1) CRL-1446
CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞
豬鼻甲黏膜成纖維細胞 PT-K75 CRL-2528
小鼠胰腺癌細胞 LTPA CRL-2389
大鼠胰腺癌細胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細胞 SP2/MIL-6 CRL-2016
大鼠腦間質(zhì)細胞 DI TNC1 CRL-2005
小鼠肌原細胞 C2C12 CRL-1772
鵪鶉?yán)w維肉瘤細胞 QT6 CRL-1708
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細胞 PL45 CRL-2558
人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03 CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
人肝癌細胞 SNU-398 CRL-2233
人成神經(jīng)瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376 CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82
人胰腺癌細胞 Capan-2 HTB-80
人胰腺癌細胞 Capan-1 HTB-79
人乳腺導(dǎo)管癌細胞 MDA-MB-435S HTB-129
人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174
人骨髓瘤細胞 U266 TIB-196
人結(jié)腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細胞瘤細胞 BE(2)-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細胞
AN3 CA 人子宮內(nèi)膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經(jīng)母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內(nèi)膜癌細胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞
BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質(zhì)母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結(jié)腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內(nèi)腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
LS 180人結(jié)腸腺癌細胞
HuTu-80十一指腸腺癌
ST豬睪丸細胞系
REH人急性非B非T淋巴細胞白血病
NCI-H520 人肺鱗癌細胞
RKO 人結(jié)腸腺癌細胞
293A 人胚腎細胞
RK-13 兔腎細胞系
李(先生) （來電時請說是從給覽網(wǎng)看到我的)
免責(zé)聲明：以上所展示的[CRL-1592 CRL-1592 IEC-6 大鼠小腸隱窩上皮細胞]信息由會員[上海復(fù)祥生物科技有限公司]自行提供，內(nèi)容的真實性、準(zhǔn)確性和合法性由發(fā)布會員負責(zé)。
