| Catalogue Number |
CYT-382 |
| Synonyms |
T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2. |
| Introduction |
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
| Description |
Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa.
The IL-2 is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein (1mg/ml) was lyophilized without any additives. |
| Solubility |
It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser. |
| Biological Activity |
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml corresponding to a specific activity of 2.5-10MU/mg. |
| Protein content |
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » IL 2 Antibody |
| » IL 2 Antibody |
| » IL 2 Human |
| » IL 2 Human, His |
| » IL 2 Mouse |
| » IL 2 Mouse, His |
| » IL 2 Porcine |
| » IL 2r Antibody |
| » IL 2r Antibody |
| » sIL 2R Human |
| » sIL 2R Human, GST |
|
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貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
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Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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