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文獻：磷酸化eIF4E結合蛋白抗體應用方法

文件類型：Word文檔
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更新日期：2022-12-02 13:51
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詳細介紹

中文名稱    磷酸化eIF4E結合蛋白抗體

英文名稱    phospho-eIF4EBP1 (Ser65 + Thr70)

別    名    eIF4EBP1 (phospho S65/Th70); eIF4EBP1 (phospho Ser65/Thr70); p-eIF4EBP1 (Ser65/Thr70); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN.

供應商    遠慕生物

產品類型    磷酸化抗體

研究領域    腫瘤  細胞生物  免疫學  信號轉導  細胞凋亡  轉錄調節因子  激酶和磷酸酶

抗體來源    Rabbit

克隆類型    Polyclonal

交叉反應    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化eIF4E結合蛋白抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量    13kDa

性    狀    Lyophilized or Liquid

濃    度    1mg/1ml

免疫原    KLH conjugated Synthesised phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65/Thr70

亞    型    IgG

純化方法    affinity purified by Protein A

儲存液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

磷酸化eIF4E結合蛋白抗體產品介紹    background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Function:
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Subunit:
Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.

Post-translational modifications:
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1.

Similarity:
Belongs to the eIF4E-binding protein family.

Gene ID:
1978

Database links:

Entrez Gene: 1978 Human

Entrez Gene: 13685 Mouse

Entrez Gene: 116636 Rat

Omim: 602223 Human

SwissProt: Q13541 Human

SwissProt: Q60876 Mouse

SwissProt: Q62622 Rat

Unigene: 411641 Human

Unigene: 6700 Mouse

Unigene: 11161 Rat

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.