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- 產品/服務：HB-198 F4/80 雜交瘤細胞 ATCC細胞
- 型 號：HB-198 F4/80
- 單 價：面議
- 更新日期：2024-09-04
- 有效期至：長期有效
- 瀏覽次數：2152
- 立即詢價
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HB-198 F4/80 雜交瘤細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和優培養條件！...
產品詳細介紹
HB-198 F4/80 雜交瘤細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和優培養條件！
HB-198 F4/80 雜交瘤細胞 的詳細介紹
HB-198 F4/80 雜交瘤細胞
ATCC® Number: HB-198™ Price: $399.00
Designations: F4/80
Depositors: S Gordon
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse macrophage
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Comments: Animals were immunized with thioglycollate elicited mouse peritoneal macrophages.
Spleen cells were fused with NS1 myeloma cells.
The antibody reacts specifically with mouse macrophages in many tissue sites (liver, spleen, bone marrow, brain), and precipitates a 160000 dalton glycoprotein (function unknown).
At the request of Dr. Gordon, the line was deposited with the ATCC in 1988 by Dr. Ralph Steinman of the Rockefeller University.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1635: Hume DA, et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med.
1636: Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed:
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上海復祥生物提供 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和優培養條件,網站上有細胞照片,聯系郵箱xiangfbio@163.com.詳情請登陸http://www.xiangbio.com/歡迎各位老師來電咨詢! | ATCC® Number: | HB-198™ | Price: | $399.00 | ||||||||||||||||||||||||||||||||||||||||||||||||
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Designations: F4/80
Depositors: S Gordon
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse macrophage
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Comments: Animals were immunized with thioglycollate elicited mouse peritoneal macrophages.
Spleen cells were fused with NS1 myeloma cells.
The antibody reacts specifically with mouse macrophages in many tissue sites (liver, spleen, bone marrow, brain), and precipitates a 160000 dalton glycoprotein (function unknown).
At the request of Dr. Gordon, the line was deposited with the ATCC in 1988 by Dr. Ralph Steinman of the Rockefeller University.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1635: Hume DA, et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med.
1636: Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed:
Related
NCBI Entrez Search
Make a Deposit
Frequently Asked Questions
Material Transfer Agreement
Technical Support
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HB-198 F4/80 雜交瘤細胞
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