CCL-75 WI-38 人胚肺細胞 的詳細介紹
CCL-75 WI-38 人胚肺細胞
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ATCC® Number:
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CCL-75?
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Price:
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$272.00
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Designations:
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WI-38
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Depositors:
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L Hayflick
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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adherent
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Organism:
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Homo sapiens (human)
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Morphology:
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fibroblast
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Source:
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Organ: lung
Disease: normal
Cell Type: fibroblast
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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CCL-75 WI-38 人胚肺細胞
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Applications:
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testing [92346] [92389]
transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
viruscide testing
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Virus Susceptibility:
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Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
Pseudorabies virus
Human poliovirus 1
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Reverse Transcript:
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negative
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DNA Profile (STR):
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Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
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Cytogenetic Analysis:
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normal diploid
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Isoenzymes:
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G6PD, B
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Age:
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3 months gestation fetus
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Gender:
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female
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Ethnicity:
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Caucasian
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Comments:
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The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue. [22194]
WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours. [22197]
This line was the first human diploid cell line to be used in human vaccine preparation. [26220]
The 8th passage ampule from which this freeze was derived was found to contain a bacterial contaminant (a micrococcus). The cell line was subsequently cured by several passages in the presence of antibiotics.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Subculturing:
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Trypsin and medium should be prewarmed to 37C and the pH should be adjusted to 7.5 before use.
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
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Preservation:
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growth medium, 95%; DMSO, 5%
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Doubling Time:
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24 hrs
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
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References:
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21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed:
22194: Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed:
22197: Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed:
26220: Hayflick L, et al. Preparation of poliovirus vaccines in a human fetal diploid cell strain. Am. J. Hyg. 75: 240-258, 1962. PubMed:
26223: . First International Conference on Vaccines against Viral and Rickettsial Diseases of Man. Pan Am. Health Organ. Off. Doc. 147: 581, 1967.
29375: Tomasek JJ, et al. Gelatinase A activation Is regulated by the organization of the polymerized actin cytoskeleton. J. Biol. Chem. 272: 7482-7487, 1997. PubMed:
32244: Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed:
32288: Landers JE, et al. Translational enhancement of mdm2 oncogeneexpressionin human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed:
32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93:
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Relatedl inks 
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NCBI Entrez Search
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Frequently Asked Questions
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Material Transfer Agreement
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Technical Support
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人胰腺癌細胞 Panc 03.27 CRL-2549
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人胰腺癌細胞 HPAF-II CRL-1997
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人臍靜脈內皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
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人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
CCL-75 WI-38 人胚肺細胞
小鼠巨噬細胞 RAW 264.7 TIB-71
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人胰腺癌細胞 Capan-1 HTB-79
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人骨髓瘤細胞 U266 TIB-196
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人多形性惡性膠質瘤 T98G [T98-G] CRL-1690
人神經母細胞瘤細胞 BE(2)-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CCL-75 WI-38 人胚肺細胞
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內膜腺癌細胞
HTB-113 HEC-1-B 人子宮內膜腺癌細胞
AN3 CA 人子宮內膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內膜癌細胞
HTB-113 hec-1-b 人子宮內膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞
BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
LS 180人結腸腺癌細胞
HuTu-80十一指腸腺癌
ST豬睪丸細胞系
REH人急性非B非T淋巴細胞白血病
NCI-H520 人肺鱗癌細胞
RKO 人結腸腺癌細胞
293A 人胚腎細胞
RK-13 兔腎細胞系
CCL-75 WI-38 人胚肺細胞CCL-75 WI-38 人胚肺細胞CCL-75 WI-38 人胚肺細胞CCL-75 WI-38 人胚肺細胞CCL-75 WI-38 人胚肺細胞
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