| Catalogue Number |
CYT-318 |
| Synonyms |
PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik. |
| Introduction |
Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes. |
| Description |
Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 348 amino acids and having 11 N-terminal flag tag The total molecular mass is 39.6 kDa (calculated). The amino acid sequence of the recombinant human Visfatin is 100% homologous to the amino acid sequence Pro27-Glu363 of the human Visfatin.
The Visfatin is purified by Flag-affinity chromatography. |
| Source |
Escherichia Coli. |
| Physical Appearance |
White lyophilized (freeze-dried) powder. |
| Formulation |
Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20mM Tris, 20mM NaCl, pH 7.5. |
| Solubility |
Add 20mM Tris, 20mM NaCl, pH 7.5 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability |
Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Visfatin should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Amino acid sequence |
MDYKDDDDKA SPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQE. |
| Applications |
Western Blot. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » Visfatin Antibody |
| » Visfatin Human, His |
| » Visfatin Mouse |
|
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Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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